New Features in 2.0.6.3.
Heat display. Functional imaging of fast calcium transients in live tissue is a very important application of two-photon microscopy. Monitoring fluorescence changes in real-time provides essential feedback on how the experiment is running. MScan allows plotting the intensity of multiple regions of interest across time even in resonant scanning mode. In release 2.0.6.3, MScan introduces a new way to visualize active areas by displaying a live heat map with “hotter” colors representing pixel intensities greater than those of a "baseline" frame. The map is superimposed over the current frame. Regions whose activity is below a pre-set threshold will be seen through the heat map. The heat map will facilitate spotting patterns of spatial activity. MView 2.0.6.3 also allows overlaying a heat map above frames, which can lead to the creation of visually appealing movies.
Data file recovery. Programs like MScan or the computer running them can crash unexpectedly, resulting in damaged data files. MView 2.0.6.3 can detect when a file generated by MScan 2.0.6.3 is corrupted and offers the possibility to repair it via a window allowing users to correct or enter missing metadata information.
Minor improvements in MScan 2.0.6.3.
- Increased limit on number of frames that can be averaged during live display or stacks. Up to 200 frames can be averaged on a rolling basis and displayed when taking movies.
- Resonant mode: frame rates of 496 fps (512 x 32 lines) and 565 fps (512 x 28 lines) have been qualified.
