Enhancement of ROI handling
Plotting the intensity of regions of interest (ROIs) in real-time, especially during resonant scanning is one of the most useful features of MScan when performing functional imaging with calcium indicators or other functional dyes. ROIs can be drawn directly on features displayed in the Viewer window as ellipses, rectangles or polygons.
With MScan 2.1, individual ROIs can be moved pixel-by-pixel with with keystrokes (Shift+1,2,3, or 4) or deleted, while all ROIs can be saved or recalled from a *.ROI file which can be used by MView to load ROIs.
ROIs are a key feature of two-photon close-loop experiments (see later post).